📘 ❞ Natural Killer Cell Protocols_ Cellular and Molecular Methods-Humana Press ❝ كتاب ــ Kerry S. Campbell Marco Colonna اصدار 2000

Biology Books - 📖 كتاب ❞ Natural Killer Cell Protocols_ Cellular and Molecular Methods-Humana Press ❝ ــ Kerry S. Campbell Marco Colonna 📖

█ _ Kerry S Campbell Marco Colonna 2000 حصريا كتاب ❞ Natural Killer Cell Protocols_ Cellular and Molecular Methods Humana Press ❝ 2025 Press: نبذه عن الكتاب: 1 Introduction Our understanding of the phenotypical functional heterogeneity of human natural killer (NK) cells has greatly advanced over past few years This advancement been helped by development culture conditions for clonal proliferation NK Analysis human cell clones has led to original observation that different clones recognize distinct major histocompatibility complex (MHC) class I specificities This prompted the production monoclonal antibodies directed surface antigens clonally distributed, and, ultimately, biochemical molecular definition of the glycoproteins functioning as MHC receptors Here we describe a limiting dilution protocol allows establishment from peripheral blood leukocytes with high efficiency 2 Materials 1 RPMI 1640 (Gibco, cat no 31870 025) + HEPES (25 mM): 42401 018) 3 Lymphocyte separation medium (LSM): (ICN Biomedicals, 50494 36427) 4 Human recombinant interleukin (IL) 2 Liquemin (25000 UI 5 mL, Roche) 5 8866 (available Dr Bice Perussia, Jefferson Medical College, Kimmel Cancer Institute, BLSB 750, 233 10th Street, Philadelphia, PA 19107, USA) 6 Antibodies: Anti CD56 (Pharmingen, 31661A, mouse IgG1), antihuman CD3 (OKT3, IgG2a, ATCC, CRL 8001), anti IgG1 phycoerythrin (PE; SBA, 1070 09), IgG2a fluorescein isothiocyanate (FITC; 1080 02) 7 Phosphate buffered saline (PBS) 8 PBS supplemented 1% fetal calf serum (FCS) Biology Books مجاناً PDF اونلاين Biologically Biology is science concerned study life, its various forms function, how these organisms interact each other surrounding environment The word biology in Greek made up two words: bio (βίος) meaning life And loggia ( λογία) means or Biology: similarity vegetation animal cover on edges African American states, existence same fossil Branches biology Biology an ancient thousands old modern began nineteenth century multiple branches Among them are: Anatomy Botany Biochemia Biogeography Biofisia Cytology science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology functions organs Taxonemia taxonomy Virology virology Zoology zoology