📘 ❞ Molecular Biology in Cellular Pathology ❝ كتاب ــ Paul G. Murray اصدار 2000
Biology Books - 📖 كتاب ❞ Molecular Biology in Cellular Pathology ❝ ــ Paul G. Murray 📖
█ _ Paul G Murray 2000 حصريا كتاب ❞ Molecular Biology in Cellular Pathology ❝ 2025 Pathology: نبذه عن الكتاب: Contents Preface xiii Preface to Histopathology xv List of Contributors xvii 1 Blotting Techniques: Methodology and Applications 1 Fiona Watson C Simon Herrington 1 1 Introduction 1 1 2 techniques 3 References 15 2 In situ Hybridisation 19 Gerald Niedobitek Hermann Herbst 2 19 2 Experimental conditions 20 2 Probes labels 23 2 4 Controls pitfalls 27 2 5 Double labelling 29 2 6 Increasing the sensitivity ISH 31 2 7 What we do our laboratories 33 2 8 ISH: examples 35 2 9 Perspective 39 2 10 40 3 DNA Flow Cytometry 49 M Ormerod 3 49 3 Definitions terms Dye used for analysis 50 3 Sample preparation 52 viii CONTENTS 3 Analysis histogram 53 3 Quality control Computer 55 3 Multiparametric measurement 57 3 Acknowledgements 59 3 59 4 Interphase Cytogenetics 61 Sara A Dyer Jonathan J Waters 4 61 4 cytogenetics 62 4 67 4 Conclusion 76 4 77 5 Oncogenes 79 Fiona Macdonald 5 79 5 Identification oncogenes Functions proto 80 5 Mechanism oncogene activation 89 5 colorectal cancer 91 5 breast 94 5 lung 95 5 haematological malignancies 96 5 Other cancers 99 5 100 5 11 100 6 Immunological Aspects Cell Proliferation 105 Karl Baumforth John Crocker 6 The cell cycle its importance clinical pathology 105 6 108 6 111 6 112 6 Immunocytochemical markers proliferating cells 115 6 133 6 Further Reading 135 7 Nucleolar Organiser Regions Tumour 137 Massimo Derenzini, Davide Trer´e, Marie Fran¸coise O’Donohue and Dominique Ploton CONTENTS ix 7 137 7 AgNORs 138 7 NOR silver staining 142 7 Quantitative AgNOR 145 7 as a parameter level proliferation 146 7 Application technique tumour 147 7 future pathology? 151 7 152 8 Apoptosis Senescence 153 Lee B Jordan David Harrison 8 153 8 senescence 174 8 Summary 178 8 179 9 Polymerase Chain Reaction 193 Timothy Diss 9 193 9 Principles 194 9 products 197 9 RT PCR 199 9 200 9 RNA extraction Correlation with morphology 201 9 Problems 202 9 Diagnostic applications 203 9 Infectious diseases 209 9 12 Identity 13 210 9 14 15 Online information 212 Books مجاناً PDF اونلاين Biologically Biology is natural science that concerned study life, various forms function, how these organisms interact each other surrounding environment word biology Greek made up two words: bio (βίος) meaning life And loggia ( λογία) means or Biology: similarity vegetation animal cover on edges African American states, existence same fossil Branches biology Biology an ancient thousands years old modern began nineteenth century This has multiple branches Among them are: Anatomy Botany Biochemia Biogeography Biofisia Cytology science Ecology environmental science Development Embryology embryology Genetics genetics Histology histology Anthropology anthropology Microbiology bacteriology Molecular Biology Physiology functions organs Taxonemia taxonomy Virology virology Zoology zoology
كتاب
Molecular Biology in Cellular Pathology
ــ Paul G. Murray
صدر 2000م كتاب
Molecular Biology in Cellular Pathology
ــ Paul G. Murray
صدر 2000م
نبذه عن الكتاب:
Contents
Preface xiii
Preface to Molecular Biology in Histopathology xv
List of Contributors xvii
1 Blotting Techniques: Methodology and Applications 1
Fiona Watson and C. Simon Herrington
1.1 Introduction 1
1.2 Blotting techniques 1
1.3 References 15
2 In-situ Hybridisation in Histopathology 19
Gerald Niedobitek and Hermann Herbst
2.1 Introduction 19
2.2 Experimental conditions 20
2.3 Probes and labels 23
2.4 Controls and pitfalls 27
2.5 Double-labelling 29
2.6 Increasing the sensitivity of ISH 31
2.7 What we do in our laboratories 33
2.8 Applications of ISH: examples 35
2.9 Perspective 39
2.10 References 40
3 DNA Flow Cytometry 49
M.G. Ormerod
3.1 Introduction 49
3.2 Definitions and terms 49
3.3 Dye used for DNA analysis 50
3.4 Sample preparation for DNA analysis 52
viii CONTENTS
3.5 Analysis of the DNA histogram 53
3.6 Quality control 53
3.7 Computer analysis of the DNA histogram 55
3.8 Multiparametric measurement 57
3.9 Acknowledgements 59
3.10 References 59
4 Interphase Cytogenetics 61
Sara A. Dyer and Jonathan J. Waters
4.1 Introduction 61
4.2 Interphase cytogenetics 62
4.3 Applications 67
4.4 Conclusion 76
4.5 References 77
5 Oncogenes 79
Fiona Macdonald
5.1 Introduction 79
5.2 Identification of the oncogenes 79
5.3 Functions of the proto-oncogenes 80
5.4 Mechanism of oncogene activation 89
5.5 Oncogenes in colorectal cancer 91
5.6 Oncogenes in breast cancer 94
5.7 Oncogenes in lung cancer 95
5.8 Oncogenes in haematological malignancies 96
5.9 Other cancers 99
5.10 Conclusion 100
5.11 References 100
6 Molecular and Immunological Aspects of Cell Proliferation 105
Karl Baumforth and John Crocker
6.1 The cell cycle and its importance in clinical pathology 105
6.2 Molecular control of the cell cycle 108
6.3 Cell cycle control 111
6.4 The cell cycle and cancer 112
6.5 Immunocytochemical markers of proliferating cells 115
6.6 References 133
6.7 Further Reading 135
7 Interphase Nucleolar Organiser Regions in Tumour Pathology 137
Massimo Derenzini, Davide Trer´e, Marie-Fran¸coise O’Donohue and
Dominique Ploton
CONTENTS ix
7.1 Introduction 137
7.2 The AgNORs 138
7.3 NOR silver-staining 142
7.4 Quantitative AgNOR analysis 145
7.5 AgNORs as a parameter of the level of cell proliferation 146
7.6 Application of the AgNOR technique to tumour pathology 147
7.7 What future for AgNORs in tumour pathology? 151
7.8 References 152
8 Apoptosis and Cell Senescence 153
Lee B. Jordan and David J. Harrison
8.1 Introduction 153
8.2 Apoptosis 153
8.3 Cell senescence 174
8.4 Summary 178
8.5 References 179
9 The Polymerase Chain Reaction 193
Timothy Diss
9.1 Introduction 193
9.2 Principles 194
9.3 Analysis of products 197
9.4 RT-PCR 199
9.5 Quantitative PCR 200
9.6 DNA and RNA extraction 200
9.7 Correlation of the PCR with morphology 201
9.8 Problems 202
9.9 Applications 202
9.10 Diagnostic applications 203
9.11 Infectious diseases 209
9.12 Identity 209
9.13 The future 210
9.14 References 210
9.15 Online information 212
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